Project Name Proteomic profiling of neutrophilic, iron-oxidizing Mariprofundus ferrooxydans, strain PV-1 
Project Groups (1)
Domain (0) Unknown
Investigators (3)
Institution N/A
Project Code 632643
Project Type

Description from C-DEBI website:
The aim of this proposal is to gain a better understanding of what subsets of proteins are actually being expressed during neutrophilic, microbial iron (Fe)-oxidation. The recently isolated Mariprofundus ferrooxydans, strain PV-1, will be used as a marine model organism to investigate proteomic differences under different Fe substrates: aqueous Fe2+ and solid Fe0. Two-dimensional gel electrophoresis (2D-GE) and shotgun proteomic methods (LC-MS/MS) will be employed to obtain results from the cultures grown under different conditions. The research being proposed would constitute the foundation for the development of diagnostic tools for the accordance, distribution, and activity level of Fe-oxidation, a globally important biogeochemical process at and below the ocean floor.

This project was funded by a C-DEBI Graduate Student Fellowship.

No assemblies.

Sample Name PI Desc Cart
Raw LC-MS/MS NA Raw LC-MS/MS data with list of identified peptides in xml format.

Related publications:
Barco, RA, Edwards, KJ. 2014. Interactions of proteins with biogenic iron oxyhydroxides and a new culturing technique to increase biomass yields of neutrophilic, iron-oxidizing bacteria. Front. Microbiol. 5:259. doi: 10.3389/fmicb.2014.00259 

Raw LC-MS/MS and DNA NA Raw LC-MS/MS data, with list of identified peptides in xml format, and DNA sequences.

Related publications:
Barco, RA, Emerson, D, Sylvan, JB, Orcutt, BN, Jacobson Meyers, ME, Ramírez, GA, Zhong, JD, Edwards, KJ. 2015. New insights into microbial iron oxidation as revealed by the proteomic profile of an obligately iron-oxidizing chemolithoautotroph. Appl. Environ. Microbiol. 81(17): 5927-37. doi: 10.1128/AEM.01374-15