Project Name Eukaryal V9 Tag Sequencing Methodology 
Domain (1) eukarya
Investigators (1)
Institution Marine Biological Laboratory, Woods Hole, Massachusetts
Project Code CAM_PROJ_ICOMM
Project Type metagenome
Publications (1)


Massively parallel pyrosequencing of amplicons from the V6 hypervariable regions of small-subunit (SSU) ribosomal RNA (rRNA) genes is commonly used to assess diversity and richness in bacterial and archaeal populations. Recent advances in pyrosequencing technology provide read lengths of up to 240 nucleotides. Amplicon pyrosequencing can now be applied to longer variable regions of the SSU rRNA gene including the V9 region in eukaryotes. We present a protocol for the amplicon pyrosequencing of V9 regions for eukaryotic environmental samples for biodiversity inventories and species richness estimation. The International Census of Marine Microbes (ICoMM) and the Microbial Inventory Research Across Diverse Aquatic Long Term Ecological Research Sites (MIRADA-LTERs) projects are already employing this protocol for tag sequencing of eukaryotic samples in a wide diversity of both marine and freshwater environments.


No assemblies.


Sample Name PI Desc Cart
ICOMM_SMPL_LTERSTATION1_REPLICATION1 NA LTERStation1 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION1_REPLICATION2 NA LTERStation1 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION2_REPLICATION1 NA LTERStation2 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION2_REPLICATION2 NA LTERStation2 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION3_REPLICATION1 NA LTERStation3 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION3_REPLICATION2 NA LTERStation3 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION4_REPLICATION1 NA LTERStation4 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION4_REPLICATION2 NA LTERStation4 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION5_REPLICATION1 NA LTERStation5 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION5_REPLICATION2 NA LTERStation5 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION6_REPLICATION1 NA LTERStation6 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION6_REPLICATION2 NA LTERStation6 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION7_REPLICATION1 NA LTERStation7 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION7_REPLICATION2 NA LTERStation7 EukV9 1380F/1389F replication2
ICOMM_SMPL_LTERSTATION8_REPLICATION1 NA LTERStation8 EukV9 1380F/1389F replication1
ICOMM_SMPL_LTERSTATION8_REPLICATION2 NA LTERStation8 EukV9 1380F/1389F replication2
ICOMM_SMPL_MHBSTATION7_1380F NA MHBStation7 EukV9 1380F
ICOMM_SMPL_MHBSTATION7_1389F NA MHBStation7 EukV9 1389F
ICOMM_SMPL_MHBSTATION7_REPLICATION NA MHBStation7 EukV9 1389F


Type Location
Meta  /iplant/home/shared/imicrobe/projects/37/CAM_PROJ_ICOMM.csv
Meta  /iplant/home/shared/imicrobe/projects/37/sample-attr.tab
Reads  /iplant/home/shared/imicrobe/projects/37/CAM_PROJ_ICOMM.read.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/739/ICOMM_SMPL_LTERSTATION1_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/740/ICOMM_SMPL_LTERSTATION1_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/741/ICOMM_SMPL_LTERSTATION2_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/742/ICOMM_SMPL_LTERSTATION2_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/743/ICOMM_SMPL_LTERSTATION3_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/744/ICOMM_SMPL_LTERSTATION3_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/745/ICOMM_SMPL_LTERSTATION4_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/746/ICOMM_SMPL_LTERSTATION4_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/747/ICOMM_SMPL_LTERSTATION5_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/748/ICOMM_SMPL_LTERSTATION5_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/749/ICOMM_SMPL_LTERSTATION6_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/750/ICOMM_SMPL_LTERSTATION6_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/751/ICOMM_SMPL_LTERSTATION7_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/752/ICOMM_SMPL_LTERSTATION7_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/753/ICOMM_SMPL_LTERSTATION8_REPLICATION1.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/754/ICOMM_SMPL_LTERSTATION8_REPLICATION2.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/755/ICOMM_SMPL_MHBSTATION7_1380F.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/756/ICOMM_SMPL_MHBSTATION7_1389F.fa
Reads  /iplant/home/shared/imicrobe/projects/37/samples/757/ICOMM_SMPL_MHBSTATION7_REPLICATION.fa