Project Name Metagenomic signatures of the Peru Margin 
Domain (1) bacteria
Investigators (1)
Institution University of North Carolina at Chapel Hill
Project Code CAM_PROJ_PeruMarginSediment
Project Type metagenome
Publications (1)


The subseafloor marine biosphere may be one of the largest reservoirs ofmicrobial biomass on Earth and has recently been the subject of debate in terms of the composition of its microbial inhabitants, particularly on sediments from the Peru Margin. A metagenomic analysis was made by using whole-genome amplification and pyrosequencing of sediments from Ocean Drilling Program Site 1229 on the PeruMargin to further explore themicrobial diversity and overall community composition within this environment. A total of 61.9 Mb of genetic material was sequenced from sediments at horizons 1, 16, 32, and 50 m below the seafloor. These depths include sediments from both primarily sulfate-reducing methane-generating regions of the sediment column.Many genes of the annotated genes, including those encoding ribosomal proteins, corresponded to those from the Chloroflexi and Euryarchaeota. However, analysis of the 16S smallsubunit ribosomal genes suggests that Crenarchaeota are the abundant microbial member. Quantitative PCR confirms that uncultivated Crenarchaeota are indeed amajormicrobial group in these subsurface samples. These findings showthat themarine subsurface is a distinct microbial habitat and is different from environments studied by metagenomics, especially because of the predominance of uncultivated archaeal groups. >


No assemblies.


Sample Name PI Desc Cart
PERUMARGINSEDIMENT_SMPL_MBSF_16AMPLIFIED NA 16 meters below seafloor
PERUMARGINSEDIMENT_SMPL_MBSF_1AMPLIFIED NA 1 meter below seafloor
PERUMARGINSEDIMENT_SMPL_MBSF_1ORIGINAL NA 1 meter below seafloor
PERUMARGINSEDIMENT_SMPL_MBSF_32AMPLIFIED NA 32 meters below seafloor
PERUMARGINSEDIMENT_SMPL_MBSF_50AMPLIFIED NA 50 meters below seafloor