Project Name Analysis of a viral metagenomic library from 200 m depth in Monterey Bay, California constructed by direct shotgun cloning 
Domain (1) viruses
Investigators (1)
Institution University of Hawaii at Manoa
Project Code CAM_P_0000788
Project Type metagenome
Publications (1)

We constructed a viral metagenome from a sample collected below the euphotic zone in a eutrophic bay of coastal California. We concentrated microbes from approximately one cubic meter of seawater collected from 200 m depth in Monterey Bay, CA using tangential flow filtration. Cellular material was depleted by differential centrifugation and filtration (0.2 ?m) and the viruses enriched, concetrated and purified by banding in a CsCl equilibrium buoyant density gradient. DNA was extracted from the virus fraction of the CsCl gradient, sheared, and cloned with no prior amplification into a plasmid vector and propagated in E. coli to produce the MBv200m library. Random clones were sequenced by the Sanger method.

No assemblies.

Sample Name PI Desc Cart
Station M1 200m NA