Sample: MMETSP0908

Sample Name MMETSP0908 
Sample Type eukaryotes
Project Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP)
Investigators (1)

Fragilariopsis kerguelensis L2-C3

Sample Accession CAM_SMPL_002742
Combined Assembly Fragilariopsis-kerguelensis-L2_C3
Taxon ID 186038

Ph 8.2  
Phosphate 1.7  
Nitrate 23.9  
Silicate 22.6  
Ammonium 0.55  
Iron 0.5  
Axenic 1  
Experimental salinity 35  
Environmental salinity 33.8  

Longhurst province SANT  

Growth medium Southern Ocean seawater  
Other environmental metadata available late austral summer to early austral fall  

NCBI SRA SRS619083  

Habitat name marine habitat  
Envo term for habitat primary term Acquatic: marine  
Envo term for habitat secondary term Not Applicable  
Habitat marine habitat  
Habitat description Atlantic sector of the Southern Ocean  

Site name Southern_Ocean  
Latitude -48.1  
Longitude -24.25  
Site description Polarstern cruise ANT-XXV/3  
Country AQ  
Depth 20  
Sample collection site Southern_Ocean  
Other collection site info Polarstern cruise ANT-XXV/3  

Project id 104  
Collection start time 2009-01-24 00:00:00.0  
Collection stop time 2009-01-24 00:00:00.0  
Volume unit L  
Filter min 20  
Clonal 1  
Collection date 24-JAN-09  
Date of experiment 30-JUN-11  
External sample id Fkerg_iron_limitation  
Investigation type EU  
Principle investigator Thomas Mock  
Primary citation Assmy, P., Henjes, J., Smetacek, V. and Montresor, M. (2006), AUXOSPORE FORMATION BY THE SILICA-SINKING, OCEANIC DIATOM FRAGILARIOPSIS KERGUELENSIS (BACILLARIOPHYCEAE). Journal of Phycology, 42: 1002–1006. doi: 10.1111/j.1529-8817.2006.00260.x  
Filter fraction minimum 20  
Volume filtered 2000  

GenBank BioSample 2740294  
Fastq file /iplant/home/shared/imicrobe/projects/104/transcriptomes/MMETSP090/MMETSP0908.fastq.tar  

Day portion of day night cycle in hours 16  
Light 80  
Experimental temperature 4  
Night portion of day night cycle in hours 8  
Environmental temperature 8.05  

Library acc CAM_LIB_001508  
Sequencing method Illumina  
Dna type cDNA  
Assembly accession number CAM_ASM_000560  
Combined assembly name Fragilariopsis-kerguelensis-L2_C3  
Pcr amp No  

Source mat id (MMETSP0908) Fragilariopsis kerguelensis - Seawater  
Source mat id 3217  
Source mat id CAM_BM_002762  
Source mat id CAM_SMPL_002742  
Sample type eukaryotes  
Sample description Fragilariopsis kerguelensis L2-C3  
Sample name MMETSP0908  
Sample volume 2000  
Taxon id 186038  
Sample material Seawater  

Strain L2-C3  
Genus Fragilariopsis  
Species kerguelensis  
Family Bacillariaceae  
Superkingdom Eukaryota  
Class Bacillariophyceae  
Phylum Bacillariophyta