Sample: MMETSP0527

Sample Name MMETSP0527 
Sample Type eukaryotes
Project Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP)
Investigators (1)

Karenia brevis SP3

Sample Accession CAM_SMPL_002816
Combined Assembly Karenia-brevis-SP3
Taxon ID 156230

Prey organism if applicable NA  

Ph 8.3  
Phosphate 36.2  
Nitrate 882  
Axenic No  
Trace elements 25  
Experimental salinity 35  

Growth medium L1 amended seawater  
Modifications to growth medium NA  
Other environmental metadata available Haplotype based on 18 microsatellite loci at  

NCBI SRA SRS618967  

Habitat name marine habitat  
Envo term for habitat primary term Acquatic: marine  
Habitat marine habitat  
Habitat description coastal harmful algal bloom  

Site name Gulf of Mexico  
Site description South Padre Island  
Country US  
Sample collection site Gulf of Mexico  
Other collection site info South Padre Island  

Project id 104  
Collection start time 1999-10-01 00:00:00.0  
Collection stop time 1999-10-01 00:00:00.0  
Clonal 1  
Collection date 01-OCT-99  
Date of experiment 08-APR-11  
External sample id SP3_35  
Investigation type EU  
Principle investigator Lisa Campbell  
Additional citations Errera, R. M., Bourdelais, A., Drennan, M. A., Dodd, E. B., Henrichs, D. W. & Campbell, L. 2010. Variation inbrevetoxin and brevenal content among clonal cultures of Karenia brevis may influence bloom toxicity. Toxicon55:195-203; Daugbjerg, N. G. Hansen,  
Primary citation Henrichs, D. W., Renshaw, M. A., Santamaria, C. A., Richardson, B., Gold, J. R. & Campbell, L. 2008. PCR amplification of microsatellites from single cells of Karenia brevis preserved in Lugol's iodine solution. Marine Biotechnology 10:122-27.  
Other experimental metadata available culture acclimated to salinity of 35  

GenBank BioSample 2740379  
Fastq file /iplant/home/shared/imicrobe/projects/104/transcriptomes/MMETSP052/MMETSP0527.fastq.tar  

Day portion of day night cycle in hours 12  
Light 70  
Experimental temperature 25  
Night portion of day night cycle in hours 12  

Library acc CAM_LIB_001582  
Sequencing method Illumina  
Dna type cDNA  
Assembly accession number CAM_ASM_000634  
Combined assembly name Karenia-brevis-SP3  
Pcr amp No  

Source mat id (MMETSP0527) Karenia brevis - seawater  
Source mat id CAM_BM_002836  
Source mat id CAM_SMPL_002816  
Sample type eukaryotes  
Sample description Karenia brevis SP3  
Sample name MMETSP0527  
Taxon id 156230  
Sample material seawater  

Strain SP3  
Genus Karenia  
Species brevis  
Family Gymnodiniaceae  
Order Gymnodiniales  
Superkingdom Eukaryota  
Class Dinophyceae  
Phylum Dinophyta